Advances in TB Detection presented at the XXVIII Transfrontier Meeting on Sensors and Biosensors
Despite significant advancements in tuberculosis (TB) diagnostics, there remains a critical need for non-sputum-based rapid tests. Our latest research, presented by Shaira Jane Acosta at the XXVIII Transfrontier Meeting on Sensors and Biosensors at IRTA, addresses this need by focusing on the selection of DNA aptamers for the highly specific recognition of the Mycobacterium tuberculosis ESAT-6/CFP-10 heterodimer antigen.
Using Capture SELEX, we successfully isolated aptamers that can recognize this biomarker in its native state. This innovative approach enhances the specificity and stability of TB detection in non-sputum samples, paving the way for more reliable and rapid diagnostics.
Key Highlights:
- Recombinantly Expressed Proteins: We used recombinantly expressed ESAT-6 and CFP-10 proteins to form the heterodimer complex.
- Library-Immobilized Selection Strategy: This strategy was employed to select aptamers against the native state of the target proteins.
- Compatibility with Human Serum: Ensured compatibility with human serum for practical application.
This breakthrough underscores the potential of aptamer-based biosensing in transforming TB diagnostics, offering a promising path towards more effective and rapid detection methods.
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